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Course: 11:115: 452 Biochemical Separations (3 cr.) This course is the same as 16:115:552 Normally Offered: (each) spring semester; Dr. William Ward (crebb@rci.rutgers.edu) Prerequisites and other registration instructions: 11:115:413 or 313 or any similar laboratory course in biochemistry. Other significant laboratory experience in biochemistry or related fields will satisfy the formal course prerequisite. Format: two 80 minute lectures per week with occasional demonstrations of separations methodologies as appropriate Description: This course is a practical introduction to methods used in separating biomolecules (proteins, nucleic acids, polysaccharides, and lipids) from each other and from other materials of biological origin. The course is built upon Dr. Ward's thirty years of continuous laboratory experience in protein purifications and separations and so it has a distinct emphasis on protein separations, generally considered to be the most difficult separations in biochemistry. Virtually all methods are covered, including: assay technologies, extractions, batch separations, column chromatography, electrophoresis, HPLC, CZE, GC, isoelectric focusing, antibody purification, criteria for judging purity, and sample preparation for protein sequencing, mass spectrometry, and related analytical techniques. Emphasis will be placed on newer methods such as separations of recombinant proteins, affinity chromatography including IMAC, three phase partitioning, and proteomics. The course is essentially non-mathematical. Not a single equation will be derived. However, the course has a firm basis in theory and "light theory" will be presented on a daily basis. The course is most useful for those with significant laboratory experience and/or ongoing projects that require biochemical separations. Subtleties and nuances of separations technology will be presented in almost every class meeting. The more you know, the more you can learn. Examinations: Two in-class exams plus a final. Nearly all exams require short answer writing. There are no multiple choice questions--never, ever, ever! Other requirements: A 10-page paper on a separations topic of interest to the student. Grading: Exams are 25%, final is 40% and term paper counts 10%. Qualitative assessment takes into consideration the student's improvements over the semester. Rarely, if ever, does a student making an A on the final get anything lower than an A in the course. Texts: None required, several recommended. Generally notes are distributed to the class in advance. Dr. William Ward The Still Assoc. Professor of Biochemistry CREBB - Centre for Research & Education in Bioluminescence & Biotechnology Rutgers University Dept. of Biochemistry & Microbiology Email:<crebb@rci.rutgers.edu> Cook College 76 Lipman Dr. Rm.216 New Brunswick NJ 08901 USA |