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    Key to practise exam 2

     

    Exam 2, total points=100, Each choice question was valued 2 points. Essay was valued at 8 points. Exam scores are posted on the Online Gradebook.

    Test Analysis- The exam was curved upwards by 6 points. With the curve the average and median were 75. The average is at the C+/B border. It is advised that you careful analyze your response to the difficult questions if you response was incorrect.

    1=E, 2=D, 3=C, 4=B, 5=C, 6=C, 7=B, 8=C, 9=C, 10=C, 11=A, 12=A, 13=D, 14=B, 15=C, 16=A, 17=B, 18=A, 19=C, 20=B, 21=A, 22=C, 23=C, 24=C, 25=B, 26=A, 27=C, 28=B, 29=B, 30=A, 31=B, 32=C, 33=C, 34=D, 35=B, 36=C, 37=B, 38=B, 39=A, 40=A, 41=B, 42=A, 43=B, 44=B, 45=B, 46=A

    Essay

    SAGE Microarray
    mRNA level is measured by sequencing cDNA tags mRNA level is measured by hybridization of cDNA to probes fixed to a slide
    the technique is laborious The technique is comparatively faster
    no hybridization so no optimization is required hybridization must be optimized
    no bias since sequence tags reveal which genes are expressed biased because an investigator chooses what probes are fixed to slide
    deep detection limit determined by the amount of sequence obtained detection limited by the hybridization signal